Assessing periodontal health status of patients with chronic periodontitis before and after non-surgical therapy (SRP) using a BANA-Enzymatic™ test kit: A clinico-microbiological study
Tanusha Sharma 1 * , Charu Agarwal 2
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1 Department of Periodontics, Ahmedabad Dental College & Hospital, Ahmedabad, Gujarat, INDIA2 Department of Periodontics, Goenka Research Institute of Dental Science, Gandhinagar, Gujarat, INDIA* Corresponding Author


Background: There are many methods available for diagnosing and assessing periodontal health. Clinical improvements after non-surgical therapy (SRP) are directly linked to the microbiological changes, which indicates a decrease in certain periodontal microflora. The BANA-Enzymatic™ test kit is one of the modern and chair side alternatives to bacterial cultures. It detects the presence of three periodontal pathogens (P. gingivalis, T. denticola, and T. forsythia) known as the red complex microorganisms and thus serve as a marker of disease activity.
Aim and objective: The aim and objective of this study was to assess periodontal health status of patients with chronic periodontitis before and after SRP using a BANA-Enzymatic™ test kit.
Materials and methods: A total number of 20 patients comprising of both the sexes with chronic periodontitis were randomly selected for the present clinical study after meeting inclusion and exclusion criteria. Each selected sites were subjected to the assessment of plaque index, bleeding index, gingival index, pocket depth, and clinical attachment level before and after three months of SRP. BANA-Enzymatic™ test kit was used for the detection of micro-organisms. Statistical analyses were done.
Results: The results are statistically correlated to the BANA test and the clinical parameters that was recorded in the study. Hence, indicating the direct correlation with the severity of periodontal disease and destruction.
Conclusion: This study encourages a simple, easy, and chair side kit– BANA-Enzymatic™ test, as for diagnosis of periodontal condition. It is also helpful identifying the microbiological flora of the plaque (presence of red complex microorganisms).


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Article Type: Original Article

ELECTR J MED DENT STUD, 2022, Volume 12, Issue 2, Article No: em0099

Publication date: 26 Jul 2022

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Article Downloads: 126

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